Editor—To try to reduce the harm caused by cigarette smoking, the European Commission established maximal values for tar (10 mg), nicotine (1 mg), and carbon monoxide (CO; 10 mg) per cigarette, as measured by the International Organization for Standardization (ISO) method, from 1 January 2004.1 The easiest way to reduce yields is by increasing filter ventilation, but this allows smokers easily to control the dose of smoke they can obtain, usually to facilitate increased intake from lower yield cigarettes.,3 We compared yields and design features of 10 cigarette brands sold in the United Kingdom before and after the EC standard was implemented.
doi: 10.1136/bmj.332.7536.302
PMID: 16455737
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Researchers at Roswell Park Cancer Institute performed analyses in September-October 2005. The 1999 brand versions had been stored unopened at room temperature since purchase; current versions were purchased in September 2005.
Full details of methods and data are available on the web (www.roswelltturc.org/research3_3.htm). Filter ventilation was assessed using a KC-3 digital apparatus (Borgwaldt-KC, Richmond, VA, USA) following a published protocol. ISO tar, nicotine, and CO yields were obtained from packs (1999 CO values are from LGC5). Wilcoxon tests assessed average changes across brands.
The table shows yields and filter ventilation for each brand. Originally rated at 11-13 mg tar (median 12 mg), all brands dropped to 10 mg tar (17% drop, P < 0.002), while reducing nicotine from a median of 1.0 mg to 0.9 mg (P < 0.008). The ratio of tar to nicotine did not change (P > 0.45). Carbon monoxide yields also dropped significantly, from a median of 13 mg to 10 mg (P < 0.01). Median ventilation increased by 479% (P < 0.006) from 1999 to 2005. None of the other design features measured showed consistent changes.
Table 1
Changes in ISO yields and ventilation for selected “full flavour” brands, United Kingdom, 1999-2005
ISO=International Organization for Standardization.
CO=carbon monoxide.
NA=CO yields were not available in LGC report.5
Our findings indicate that manufacturers complied with the EC's recent mandated yield reduction primarily by increasing filter ventilation rates on cigarettes—a design feature that promotes compensatory smoking. The current “10-1-10” standard is therefore unlikely to reduce smoke exposure for smokers.3 The EC, while recognising the compensation problem, has said that it will not revise the standard until solid evidence shows that better methods exist.1
Our data suggest that removing erroneous yields from packs and adopting alternative approaches to reducing the harmfulness of cigarettes, such as banning filter vents, seems warranted. However well intentioned the EC's effort to make cigarettes less harmful, focusing solely on the use of maximum yields has served to promote increased levels of filter ventilation, which is both ineffective and misleading.
Notes
Contributors: Susan Anderson provided the cigarettes for testing. Tammy Vance performed the cigarette measurements.
Funding: This work was performed under a Transdisciplinary Tobacco Use Research Center grant to Roswell Park Cancer Institute from the US National Institutes of Health (1 P50 CA111236).
Competing interests: KMC and LTK have provided expert testimony in court cases against the tobacco industry. KMC received travel expenses for speaking at a tobacco litigation seminar. RJO, AM, and GAG have no competing interests to declare.
References
1. Report from the Commission to the European Parliament, the Council and the European Economic and Social Committee. First report on the application of the tobacco products directive. Brussels: Commission of the European Communities, 27 July 2005.
2. Kozlowski LT, O'Connor RJ. Filter ventilation is a defective design because of lighter taste, bigger puffs, and blocked vents. Tob Control 2002;11(suppl 1): i40-50. [PMC free article] [PubMed] [Google Scholar]
3. US National Cancer Institute. Risks associated with smoking cigarettes with low tar machine-measured yields of tar and nicotine. Bethesda, MD: US Department of Health and Human Services, National Institutes of Health, National Cancer Institute, 2001.
4. Kozlowski LT, Mehta NY, Sweeney CT, Schwartz SS, Vogler GP, Jarvis MJ, et al. Filter ventilation and nicotine content of tobacco in cigarettes from Canada, the United Kingdom, and the United States. Tob Control 1998;7: 369-75. [PMC free article] [PubMed] [Google Scholar]
Cigarette Tar And Nicotine Content
5. LGC (Teddington). Tar, nicotine and carbon monoxide yields of cigarettes. Tables from Survey 43—January to December 1999. www.ash.org.uk/html/regulation/html/tartables.html (accessed 18 Jan 2006)
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Addict Health. 2012 Winter-Spring; 4(1-2): 28–35.
PMID: 24494133
This article has been cited by other articles in PMC.
AbstractBackground
There are many different kinds of cigarettes and tobacco available in the market. Since nicotine content of various brands of cigarettes are very variable, therefore evaluation and comparison of nicotine content of different brands of cigarettes is important. The goal of the present study was to determine and compare nicotine content of various domestic and imported cigarettes available in the area.
Methods
Fourteen popular imported brands and nine popular domestic brands of cigarettes and three available brands of tobaccos were investigated for the amounts of nicotine content. Nicotine was extracted from each cigarette and tobacco samples and was analyzed by high performance liquid chromatography (HPLC) method.
Findings
The amount of nicotine in each cigarette was from 6.17 to 12.65 mg (1.23 ± 0.15 percent of tobacco weight in each cigarette) in domestic cigarettes. It was between 7.17-28.86 mg (1.80 ± 0.25 percent of tobacco weight in each cigarette) for imported cigarette, and between 30.08- 50.89 mg (3.82 ± 1.11 percent) for the pipe nicotine. There was significant difference in nicotine amount between imported and domestic brands of cigarettes. There was also no significant difference in nicotine content between light and normal cigarettes in imported brands.
Conclusion
Nicotine content of all tested cigarettes, imported and domestic brands, were higher than the international standard.
Keywords: Nicotine, Tobacco, Cigarettes, Human health, Brand
Introduction
Using tobacco is one of the leading preventable causes of disease and premature death worldwide. Tobacco use contributes in 10 global deaths and is the second major cause of mortality in the world.,2 There are approximately 1.25 billion smokers in the world representing about a third of the adult global population; 800 million of these people live in developing countries. While the cigarette consumption has been increased in most of the developing countries, the past 25 years has been marked by a steady decline in cigarette consumption in some developed countries. However the world cigarette production has increased about four times during the last 50 years.3-7 It has been reported that to date, more than 3000 chemicals have been isolated from tobacco which more than 1000 of these chemical constituents present in unburnt.8-9
Nicotine is the major active molecule in cigarette smoke. It is an alkaloid present in the leaves of Nicotiana tabacom. It is colorless, highly volatile alkaloid. In large doses nicotine is highly toxic. Major symptoms of nicotine poisoning are sweating, vomiting, mental confusion, diminished pulse rate and breathing difficulty. People who smoke have more chronic illnesses, including emphysema and bronchitis, cardiovascular disease, cancer, bronchopulmonary disease, etc.-15 There are many brands of cigarette (domestic and imported) available in Iranian market. Therefore, it seems important to measure the amount of nicotine in different kinds of cigarettes which is generally used by the people in this country.
Most of the imported cigarettes have labeled with the nicotine yields and many consumers are highly motivated to select cigarettes with lower nicotine yields for their health benefits. However, the nicotine level rating on the cigarette label (12 mg tar and 1 mg nicotine in a low-yield cigarette) is not the same as the total amount of nicotine present in the cigarette., The “nicotine yield” is determined by a smoking machine; a syringe which draws 32 ml puffs each minute unit the total length of a cigarette is burned. On the other hand, measuring the total amount of nicotine that exists in the cigarette is called “nicotine content”.
Since nicotine is the major compound in cigarettes and it is highly toxic, knowing the amount of nicotine content in cigarettes can be valuable information for the people smoking cigarettes. In this project the amount of nicotine content of various popular brands of the imported and domestic cigarettes available in the Iranian market was investigated.
Methods
Pure nicotine was obtained from Fluka, Switzerland. All other solvents and chemicals were of analytical grade and obtained from Sigma Aldrich GmbH. Sternheim. Germany.
Cigarette and tobacco products
Twenty two different brands of cigarettes, nine popular domestic brands of cigarette (made in Iran) and thirteen popular brands of the imported cigarettes, available in the market were chosen to evaluate their nicotine content (Table 1). All brands were filter cigarettes except for one domestic brand which was non-filter cigarette. Furthermore, three available and popular imported pipe tobaccos (Captain Black Cherry, Captain Black Royal, Captain Black Gold) were also investigated for their nicotine contents. All the cigarette and tobacco samples were obtained from the market.
Table 1
The name of the popular domestic and imported brands of cigarettes that were studied
Extraction procedure
Four pack of each brand of cigarette was chosen randomly and one cigarette from each pack was taken to test. Before any extraction, papers and filters of the cigarettes were removed and the amount of tobacco in each cigarette was weighed. The tobacco of each cigarette was crashed carefully in a blender for one minute. The crashed tobacco of each cigarette was quantitatively suspended in 100 ml of mixture of methanol: 0.1 N NaOH (1:1) solutions. The mixture ultrasonically vibrated for 1 hour and then centrifuged for 10 minutes. To 1 ml of the supernatant, 2 ml of metronidazole solution (0.2 mg/ml, using H2O as solvent, as internal standard) was added and the total volume was made up to total of 10 ml, using 0.01 M phosphate buffer (pH 7.0) solution. From this solution, 20 µl were injected into the HPLC (n = 3). In orders to measure the nicotine quantity of three different popular pipe tobacco, five grams of each tobacco were placed in a glass plate and left at 70°C in an oven for 60 minutes to dry. The dried tobacco was weighed again and the amounts of moisture in each brand of tobacco were estimated. However, the same extraction procedure described for cigarette was used to extract nicotine from pipe tobaccos and a solution was prepared for injection into the high performance liquid chromatography (HPLC). The test was not blinded to the brands and all the analysis were done in triplicate.
Chromatographic conditions
There are several analytical methods available for measuring nicotine in cigarette.- In the present study one of the published HPLC methods according to the laboratory condition was chosen and applied for measuring nicotine in cigarettes after some modifications. The HPLC system consisted of a pump (Model 600E, waters), a variable wavelength detector (Model 484, waters), a U6K injector and a recorder (Model 745B, waters). The HPLC column was a reverse phase C18 column (4 µm, 150 ´ 4.6 mm i.d., Nova pack, Waters) operated at ambient temperature (25 ± 1 °C) in an air conditioned room. The mobile phase was consisted of 12% acetonitrile in 0.01 M phosphate buffer at a flow rate of 1 ml/min. Concentrated orthophosphoric acid was used to adjust the pH of the mobile phase to 7.0. The mobile phase was then filtered and degassed before use, using a vacuum filter system equipped with 0.45 mm filter membrane. The absorbance was monitored at 261 nm. The retention time for nicotine and metronidazole were 6.42 and 2.95 minutes, respectively. No interfering peaks from tobacco extract were observed. Nicotine concentration was calculated using peak area ratio of internal standard and sample peak. Nicotine content was expressed as the concentration of nicotine in tobacco and also as the total amount of nicotine in one entire cigarette.
Standard Solutions
Stock solution (0.2 mg/ml) of nicotine and metronidazole (internal standard) were prepared by dissolving accurately weighed quantities of pure compounds separately in distilled water. The stock solution remained stable for more than a month when stored at -20°C. Working standard solutions of nicotine (different concentrations of 10, 15, 20, 23 and 30 µg/ml) were prepared by dilution of the stock solution with distilled water. To 1 ml of each standard sample, 2 ml of internal standard was added and the volume made up to 10 ml with distilled water as for the test samples. These standard samples were also injected to the HPLC. The standard solutions of nicotine were freshly prepared daily prior to use.
Results
The standard samples were used to evaluate the method of analysis. Before the analysis of the nicotine samples which were extracted from different brands of cigarette, the method was evaluated. Calibration curve were constructed by plotting peak height ratios of nicotine to internal standard against the respective concentrations. The standard curve over the range of 0 to 30 µg/ml was linear. Intra- and inter-day variations were assessed at 10, 20 and 30 µg/ml. The coefficients of variation were between 1.9% and 4.3%.
The amount of nicotine and percentage of nicotine in each cigarette, in thirteen imported brands and nine brands of domestic cigarette are presented in tables 2 and and3,3, receptively. Percentage and amount of nicotine in three tested pipe tobacco were also evaluated and the findings are presented in table 4.
Table 2
Amounts of nicotine and tobacco as well as percentage of nicotine content in each cigarette in domestic brands*
*Four pack of each brand of cigarette was randomly chosen and one cigarette from each pack was taken for measurements
Table 3
Amounts of nicotine and tobacco as well as percentage of nicotine content in each cigarette of popular imported brands*
*Four pack of each brand of cigarette was randomly chosen and one cigarette from each pack was taken for measurements
Table 4
Amounts of nicotine and percentage of nicotine content in of one gram of popular imported brands of pipe tobacco
Cigarettes contained an average of 1.80 ± 0.25 (mean ± SD), 1.23 ± 0.15 and 3.82 ± 1.11 percentage of nicotine for the imported brands of cigarettes, the domestic brands of cigarettes and imported pipe tobaccos, respectively. Average amount of nicotine and tobacco in one entire cigarette of imported brands were 13.41 ± 4.81 mg (ranged 7.17-28.86 mg), and 0.80 ± 0.49 g (ranged 0.38-2.48 g), respectively. They were 0.28 ± 1.90 mg (ranged 6.17-12.65 mg) and 0.77 ± 0.14 g (ranged 0.45-0.93 g) for the domestic brands of cigarettes, respectively (Table 2 and and3).3). However, the average amount of nicotine in one gram of different imported brands of pipe tobacco was 38.17 ± 11.15 mg (ranged 30.08-50.89 mg).
Variation in the percentage of nicotine between the tested domestic and imported brand of cigarettes as well as between light and ordinary tested cigarettes were shown in figures 1 and and2,2, respectively. In addition, the percentage of nicotine content variation among all the testes light cigarettes were compared in figure 3.
Percentages of nicotine in different domestic and imported brands of cigarettes (Mean ± SD)
Comparison between percentages of nicotine in light and ordinary cigarettes (Mean ± SD)
Comparison between percentages of nicotine in different light cigarettes (Mean ± SD)
Discussion
Thirteen popular brands of imported and nine popular brands of domestic cigarettes as well as three brands of imported pipe tobacco were analyzed for their amount and percentage of nicotine content. Accordingly, cigarettes brand “57” contained the lowest amounts of nicotine and “Mehr” contained the highest amounts of nicotine. Although the amounts of nicotine in these cigarettes were significantly different but the percentage of nicotine in both of these cigarettes were about the same. Statistical analysis showed that there was a statistically significant difference in the amounts and percentage of nicotine between cigarettes randomly chosen from four different packs of each domestic brand.
Among the imported brands, cigarette “pine” contained the lowest amounts of nicotine and cigarette “Winston” contained the highest amounts of nicotine (14.40 mg). The percentage of nicotine in these imported brands was different. Statistical analysis showed that in imported brands, there was a significant difference in the amounts and percentage of nicotine between the cigarettes randomly chosen from four different packs of each brands.
Although the amount of tobacco in domestic and imported cigarettes was not significantly different, but there were significant differences in nicotine balance (amount and percentage of nicotine) between domestic and imported cigarettes. In all the available tested cigarettes, the amount of nicotine and tobacco widely varied based on their length and size, which determined the amounts of tobacco in each cigarette. Therefore, the amount of nicotine and tobacco in different brands depended on the way they were made.
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Considering the amounts of tobacco in each cigarette and evaluating the percentage of nicotine in domestic brands, the highest percentage of nicotine were found in “Bahman” cigarette and the in “Shiraz” cigarette. For imported brands the highest percentage of nicotine were found in “Winston light” cigarette and the lowest in “Mond light” cigarette. There has been many reports and research about nicotine and tobacco.7,, In addition, there has been some attempt to introduce smokeless tobacco product by cigarette industry. The large variation in the levels of some toxicants and carcinogens in these products indicates that there is more effort needed to reduce the amounts of these toxic compounds in the new and traditional smokeless tobacco products.
Some of the researches are about the evaluation of the nicotine content of cigarettes available in the market. A report from Japan indicates the determination of nicotine content in popular cigarettes. In this report sixteen domestic and seventeen imported brand of cigarette were studied. One of the brands (Kent) tested in Japan were the same as what we analyzed in Iran. The average amounts of nicotine were 11.24 and 14.61 mg, and percentage of nicotine were 1.71% and 1.80% in each of these cigarette analyzed in Japan and in Iran, respectively.
It is clear that in all the available cigarettes, the amount of nicotine and tobacco widely varied according to their length and size, which determine the amounts of tobacco in each cigarette. Therefore, the amount of nicotine and tobacco not only in different brands but even for one brand depends on the way they were made. Among the imported tobacco, the “Captain Black Gold” brand had the lowest percentage of nicotine while the brand “Captain Black Cherry” had the highest percentage of nicotine.
Some of the imported cigarettes were labeled as “light”. Considering the amount of tobacco as well as amount and percentage of nicotine, no significant differences were found between normal and light cigarettes. The main differences between them, if there was any, probably depended on the way they made as well as the length and size of the cigarettes.
Conclusion
Finally, it can be concluded that the average amount (as well as the percentage) of nicotine in domestic cigarettes are lower in comparison to the imported one. Considering findings of the present study and other published data as well as the highly addictive psychoactive characteristic of nicotine in tobacco products, it is suggested that nicotine be thoroughly decreased in these products as low as possible in order to reduce the chance of damages to human health caused by long-time cigarette smoking. Although the best way and the only safe and effective way to minimize smoking related health risks is to avoid smoking.
Acknowledgments
The authors would like to thank the authorities in Mashhad University of Medical Sciences and in School of Pharmacy (MUMS) for their supports. The results described in this study were part of a Pharm. D. degree thesis.
Footnotes
Conflicts of Interest
The Authors have no conflict of interest.
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Capstan is a British brand of unfiltered cigarettes, currently owned and manufactured by Imperial Brands. The brand dwindled in popularity when the health effects of tobacco became more widely known; few shops sell them today.
History[edit]
An old pack of Capstan cigarettes, c. 1930
Capstan was originally launched by W.D. & H.O. Wills in 1894, and was one of the most popular brands of cigarettes in the early-twentieth century.[1] W.D. & H.O. Wills spent £4,000 (equivalent to £477,509.50 in 2018) on promoting the Capstan cigarettes in 1900, and these amounts were in addition to regular charges for advertising, including showcards and newspaper advertisements.[2] It was W.D. & H.O. Wills' answer to Player's Medium cigarettes.[3] In 1973, the UK Government published a table of the tar and nicotine contents of cigarettes available in the UK market, and Capstan Full Strength contained, by a margin of 0.21 mg/cigarette, the highest nicotine content (3.39 mg/cigarette) of any brand, and the second-highest tar content.[4][5] However, since 2004 cigarettes sold in the UK have had a cap of 10 mg of tar and 1 mg of nicotine per cigarette.
Various advertising posters were made for Capstan cigarettes, including one to encourage female workers in factories during World War II to smoke Capstan to relax at the end of a working day. One of the most well-known slogans at the time was 'Time for a Capstan'.[6][7][8] Another popular slogan after the end of WWII was 'Have a Capstan'.[9][10] A few celebrities advertised this brand, such as English actress Evelyn Laye and British Hollywood star David Niven.[11]
The song 'Saturday's Kids' from The Jam's 1979 album Setting Sons features the lyric 'Their mums and dads smoke Capstan non-filters/Wallpaper lives 'cause they all die of cancer'.
Markets[edit]
Capstan is mainly sold in the United Kingdom, but also was or still is sold in Germany, Sri Lanka, India, Pakistan, Malaysia, Hong Kong, Australia, New Zealand, Canada and Chile.[12][13][14]
Cigarette cards[edit]
Capstan was one of the tobacco companies to include advertising cards in their packs of cigarettes. Some of the collections featured were the cricket series featuring notable players (1907)[15] and the Australian rules football collections that includes depictions of club flags and colors in 1908 and 1913.[16]
References[edit]
External links[edit]
Retrieved from 'https://en.wikipedia.org/w/index.php?title=Capstan_(cigarette)&oldid=879441199'
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